Molecular Diagnostics - CVM - VDL, University of Minnesota
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  Home > Services and Fees > Guide and Fees for Specific Tests and Procedures > Molecular Diagnostics
 

Molecular Diagnostics

 

Effective August 18, 2008

TEST 

FEE
(US $)

SPECIMEN 

SHIPPING CONTAINER 

COOLANT 

DAYS TESTED 

TURN-AROUND 

COMMENTS 

Actinobacillus pleuropneumoniae - APP
31.50
1) tonsillar swab
2) whole tonsil
3) lung
1) swab
2,3) tissue
frozen gel packs M 2-10 days Results reported on Tuesdays. Samples should be received by the previous Friday.

Additional info.
Actinobacillus pleuropneumoniae - APP Serotypes 3, 6 and 8
31.50
1) tonsillar swab
2) whole tonsil
3) lung
1) swab
2,3) tissue
frozen gel packs M-F 2-10 days Testing upon request.
Actinobacillus pleuropneumoniae - APP Serotyping
31.50
bacterial isolate culture media frozen gel packs T 2-10 days

Results reported on Wednesdays. Samples should be received by the previous Monday. (Rush testing available upon request)

Actinobacillus suis
31.50
1) tonsilllar swab
2) whole tonsil
3) lung

1) swab
2,3) tissue

frozen gel packs M 2-10 days

Results reported on Tuesdays. Samples should be received by the previous Friday.

Additional info.

Avian Pneumovirus - APV

5.25

tracheal swabs

15 ml screw cap vial (5 swabs/vial)

frozen gel packs

once monthly

up to 4 weeks

10 swabs used per test.
Call lab for testing schedule.

Bacillus anthracis
no charge

1) bacterial cultures
2) blood
3) tissue

1) leakproof container
2) tube
3) whirl pak or plastic bag

frozen gel packs as requested 7 days Testing is completed at the MN Dept of Health
Bacterial identification by DNA sequencing (16S rRNA gene)
26.25
bacterial isolate culture media frozen gel packs Th 1-2 weeks

Results reported on Thursdays.

Additional info.

Bovine Viral Diarrhea - BVD

1) 5.25
2) 14.50

1) serum
2) tissue

1) leakproof 1.5 ml microfuge tube
2) whirl pac or plastic bag

frozen gel packs

M,W

next day (from test date)

1) Submit at least .5 ml.

Bovine Viral Diarrhea - BVD ear notch screen
pooled testing

(See Virology for BVD ear notch individual testing)
26.25
1cm x 1cm ear tissue
(1/2 inch notching pliers)
6 or 10 ml red top tube (no additive or gel) frozen gel packs M,W 1-2 days

Samples will be run in pools up to 50.

Additional info.

Bovine Viral Diarrhea - BVD Genetic Analysis
126.00
1) serum
2) virus isolate
3) tissue
1,2) 1.5 ml microfuge tube
3) whirl pac or plastic bag
frozen gel packs weekly 2-4 weeks 1,2) Need a minimum of 300uL sample.
2) Must come with a copy of an USDA shipping permit.
3) 1 cm3 or biopsy. Shipping instruction: send next day delivery.

Bovine Viral Diarrhea - BVD, individual or pooled samples
(for Alpacas and Llamas only)

26.25
(individual)

5.25
(pool)

1) serum
2) tissue
3) buffy coat

1) leakproof 1.5 ml microfuge tube
2) whirl pac or plastic bag
3) 3-5 ml purple top whole blood tube

frozen gel packs

M,W

next day

Results will be reported as individual animals.

Brachyspira hyodysenteriae and Brachyspira pilosicoli multiplex PCR  

31.50

bacterial isolate

culture media

frozen gel packs

M

2-10 days

Results are reported on Tuesdays.

Circovirus Universal (Types 1 and 2)  

37.50

1) serum
2) tissue
3) feces

1) leakproof 1.5 ml microfuge tube
2,3) whirl pac or plastic bag

frozen gel packs

T,Th

1-2 days (from test date)

1) Submit at least .5 ml.

Circovirus Type 2

31.25

1) semen
2) serum
3) tissue
4) blood swabs
5) feces

1,4) leakproof 1.5 ml microfuge tube
2) red top tube
3,5) whirl pac or plastic bag

frozen gel packs

T,Th

1-2 days (from test date)

1,2,4) Submit at least .5 ml.

Circovirus Type 2 Genetic Analysis
126.00
1) serum
2) virus isolate
3) tissue
1,2) 1.5 ml microfuge tube
3) whirl pac or plastic bag
frozen gel packs weekly 2-4 weeks 1,2) Need a minimum of 300uL sample.
2) Must come with a copy of an USDA shipping permit.
3) 1 cm3 or biopsy. Shipping instruction: send next day delivery.

Clostridium perfringens, toxin gene typing

28.50

bacterial isolate

culture media

frozen gel packs

T

2-10 days

Results reported on Wednesdays. Sample should be received by the previous Monday. Submission of pure isolate requires USDA permit.

Equine encephalidites

no charge

brain tissue

   

daily

4 days

Sent to Minnesota Department of Health.

Erysipelothrix rhusiopathiae
31.50

1) skin lesions
2) systemic tissues
3) fetuses

1) tissue frozen gel packs M 2-10 days Results reported on Tuesdays. Samples should be received by the previous Friday.

Additional info.

Escherichia coli Virulence Factors

27.75

bacterial isolate

culture media

frozen gel packs

W

2-10 days

Results reported on Thursdays. Samples should be received by the previous Tuesday. Submission of pure isolate requires USDA permit.

Exotic Newcastle Disease Matrix (USDA)

24.25

tracheal swabs

synthetic swabs

frozen gel packs

W

1-2 days (from test date)

Swabs may be pooled up to 5.

Exotic Newcastle Disease Fusion (USDA)

12.75

tracheal swabs

synthetic swabs

frozen gel packs

W

1-2 days (from test date)

Swabs may be pooled up to 5. Fusion PCR is run to detect virulent NDV after a positive is obtained by END virus Matrix PCR testing.

Freemartin test in cattle

36.25

3-5 ml whole blood

purple top (EDTA) tube (preferred) or green top (heparin) tube

frozen gel packs

F

2-7 days (from test date)

Shipping instruction: send next day delivery.

Genotype analysis, bacterial (BioNumerics)
31.50
bacterial isolate culture media
frozen gel packs W,Th 1-2 weeks Results reported on Tuesdays. Submission of pure isolate requires USDA permit.
Haemophilus parasuis
31.50
systemic tissues (fibrin)

1) swab
2) tissue
3) fluid

frozen gel packs M,Th 2-10 days Results reported on Tuesdays and Fridays.

Additional info.
Influenza virus group A,
Subtyping for H & N
18.25
1) nasal swabs
2) virus isolate 3) lung

1) synthetic swab
2) leakproof 1.5 ml microfuge tube
3) whirl pac or plastic bag

1,3) frozen gel packs
2) on dry ice
W 1-2 weeks

Subtyping tests are run on samples previously testing positive for influenza virus.
2) Submit at least .5 ml. Must come with a copy of an USDA shipping permit.
3) 2-3 cm3 or biopsy. Shipping instruction: send next day delivery.

Influenza virus group A,
Subtyping for H5/H7 (USDA)
12.75
swabs synthetic swabs frozen gel packs M,W 24 hrs. (from test date) Testing for subtyping is run after a positive is obtained by Influenza virus Matrix testing.

Influenza virus, group A Matrix RRT-PCR

24.25

1) nasal swabs
2) lung

1) synthetic swab
2) whirl pac or plastic bag

frozen gel packs

M,T,W,F

2-3 days

2) 2-3 cm3 or biopsy. Shipping instruction: send next day delivery.

Influenza virus, group A Matrix (USDA)*

*for avians

24.25
swabs synthetic swab frozen gel packs M,T,W,F 2 days Swabs may be pooled up to 5. Results reported same day as test date.

Influenza virus sequencing

126.00

1) nasal swabs
2) viral isolate
3) lung

1) synthetic swab
2) leakproof 1.5 ml microfuge tube
3) whirl pac or plastic bag

1,3) frozen gel packs
2) on dry ice

weekly

2-4 weeks

2) Submit at least .5 ml. Must come with a copy of an USDA shipping permit.
3) 2-3 cm3 or biopsy. Shipping instruction: send next day delivery.

Largemouth Bass virus
25.00
1) tissue  
2) culture media
sterile container frozen gel packs M-F 3-5 days  
Lawsonia intracellularis
30.00

1) ileum
2) feces
3) swabs (fecal or rectal)

1,2) sterile container
3) synthetic swab
frozen gel packs M-F 7 days  
Leptospira sp.
15.75
1) 10 ml urine
2) tissue (fetuses, kidney)
3) 100 ml water

1) vial
2) plastic bag
3) leakproof container

frozen gel packs W 2-10 days Results reported on Fridays.
Mycobacterium paratuberculosis (Johne's)

20.00

feces (no more than 10g needed)

Do NOT use GLOVE or WHIRL PAC BAG as container. Use leakproof hard plastic container with screw cap.

frozen gel packs or dry ice

M,T,F

3 days-1 week

Please make sure container has no outside contamination. NO GLOVES or WHIRL PAC BAGS. Specimens from sheep, goat and exotic ruminants also accepted.

Mycoplasma hyopneumoniae

25.25

1) swabs (nasal, tracheal, or bronchial)
2) lung

1) synthetic swab
2) whirl pac or plastic bag

frozen gel packs

M,Th

next day (from test date)


Mycoplasma hyopneumoniae Genetic Analysis (P146 Adhesin sequencing)

126.00

1) swabs (nasal, tracheal, or bronchial)*
2) lung*
3) isolate

*Positive for M. hyo. by PCR

1) synthetic swab
2) whirl pac or plastic bag
3) sterile tube

frozen gel packs

weekly

2-4 weeks

1) Extracted DNA can be sent in FTA paper for testing.
2) Must come with a copy of USDA shipping permit.
3) Shipping instruction: send next day delivery

Mycoplasma hyorhinis

15.75

1) joint swab
2) joint fluid
3) synovial membrane
1) swab
2) tissue
frozen gel packs M 2-10 days

Results reported on Tuesdays.

Mycoplasma hyosynoviae

15.75

1) joint swab
2) joint fluid
3) synovial membrane
1) swab
2) tissue
frozen gel packs M 2-10 days

Results reported on Tuesdays.

Mycoplasma speciation

26.25

bacterial isolate culture media frozen gel packs Th 1-2 weeks

Results reported on Thursdays.

Additional info.

Myxobolus cerebralis
26.25
1) tissue  
sterile container frozen gel packs M-F 3-5 days  

Pasteurella multocida,
Toxin Gene

31.50

1) nasal swabs
2) P. multocida isolate

1) swab
2) culture media

frozen gel packs

M

2-10 days

Results reported on Tuesdays. (Rush testing available)

Porcine Cytomegalovirus - PCMV

52.50

1) nasal turbinates
2) nasal swabs
3) buffy coat

 

frozen gel packs

M

2-7 days (from test date)

Samples should be received by the previous Friday.

Porcine Parvovirus - PPV

Ref. lab fee

1) fetal thoracic fluid
2) tissue

1) leakproof container
2) whirl pac or plastic bag

frozen gel packs

 

 

Sent to reference lab.

Porcine Reproductive and Respiratory Virus -
NAEU PRRS*

24.75

1) semen
2) serum
3) tissue
4) blood swabs
5) nasal swabs
6) saliva

1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl pac or plastic bag
5) synthetic swab
6) sterile tube or whirl pac

frozen gel packs

M-F

boar stud monitoring-same day; regular request-next day

Holiday schedule for boar stud monitoring

1) At least 1 ml sample needed.
2) Submit at least .5 ml.
3) 1 cm3 or biopsy. Shipping instruction: send next day delivery.

Additional info.

Porcine Reproductive and Respiratory Virus-
NAEU Differential PRRS*

24.75

1) semen
2) serum
3) tissue
4) blood swabs
5) nasal swabs
6) saliva

1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl pac or plastic bag
5) synthetic swab
6) sterile tube or whirl pac

frozen gel packs

weekly

1-7 days

1) At least 1 ml sample needed.
2) Submit at least .5 ml.
3) 1 cm3 or biopsy. Shipping instruction: send next day delivery.

Additional info.

Porcine Reproductive and Respiratory Virus - PRRS, quantitative (U.S. strain only)

30.00

1) semen
2) serum
3) tissue

1,2) 1.5 ml microfuge tube
3) whirl pac or plastic bag

frozen gel packs

T

1-2 days (from test date)

1) At least 1 ml sample needed.
2) Submit at least .5 ml.
3) 1 cm3 or biopsy. Shipping instruction: send next day delivery.

Additional info.

Porcine Reproductive and Respiratory Virus - PRRS, Genetic Analysis (Strain differentiating/sequencing
126.00
1) serum
2) virus isolate
3) tissue
4) extracted RNA
1,2) 1.5 ml microfuge tube
3) whirl pac or plastic bag
4) sterile tube
frozen gel packs weekly 2-4 weeks

1,2) Need a minimum of 300uL sample.
2) Must come with a copy of an USDA shipping permit.
3) 1 cm3 or biopsy. Shipping instruction: send next day delivery.

Additional info.

Renibacterium salmoninarum
25.00
1) tissue  
2) culture media
sterile container frozen gel packs M-F 3-5 days  

Salmonella enterica Real-time PCR (TaqMan®)

26.25

1) tissue
2) feces (no more than 10g needed)

Do NOT use GLOVE or WHIRL PAC BAG as container.

Use leakproof hard plastic container with screw cap

frozen gel packs or dry ice

Th

3-7 days

Please make sure container has no outside contamination. NO GLOVES or WHIRL PAC BAGS.

Spring Viremia of Carp
25.00
1) tissue  
2) culture media
sterile container frozen gel packs M-F 3-5 days  

Streptococcus suis PCR

31.50

systemic tissues

1) swab
2) tissue
3) fluid

frozen gel packs

F

2-10 days

Results are reported on Mondays.

Streptococcus suis serotyping PCR (serotypes 1,2,7 and 9)  

31.50

bacterial isolate

culture media

frozen gel packs

M

2-10 days

Results are reported on Tuesdays.

Tetracore PRRS Multiplex PCR

26.25

1) semen
2) serum
3) tissue
4) blood swabs

1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl pac or plastic bag

frozen gel packs

W

1 day from test day

1) At least 1 ml sample needed.
2) Submit at least .5 ml.
3) 1 cm3 or biopsy. Shipping instruction: send next day delivery.

Additional Info.

Tetracore European

26.25

1) semen
2) serum
3) tissue
4) blood swabs

1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl pac or plastic bag

frozen gel packs

W

1 day from test day

1) At least 1 ml sample needed.
2) Submit at least .5 ml.
3) 1 cm3 or biopsy. Shipping instruction: send next day delivery.

Additional Info.

Tetracore US

26.25

1) semen
2) serum
3) tissue
4) blood swabs
1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl pac or plastic bag
frozen gel packs W 1 day from test day 1) At least 1 ml sample needed.
2) Submit at least .5 ml.
3) 1cm3 or biopsy. Shipping instruction: send next day delivery.

Additional Info.

Transmissable Gastroenteritis Virus - TGEV

26.25

1) feces
2) fecal swab
3) fresh small intestine
1,3) whirl pac or leakpoof container
2)swab
frozen gel packs      
Viral Hemorrhagic Septicemia
25.00
1) tissue  
2) culture media
sterile container frozen gel packs M-F 3-5 days RT-PCR or qRT-PCR
Yersinia ruckeri
26.25
1) tissue  
2) culture media
sterile container frozen gel packs M-F 3-5 days  

* The Veterinary Diagnostic Laboratory prefers pooling samples at the lab.
** Samples with low levels of virus may need to be expanded by virus isolation.

 


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