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  Home > Services and Fees > Guide and Fees for Specific Tests and Procedures > Bacteriology
 

Bacteriology

 

TEST 

FEE
(US $)  

SPECIMEN 

SHIPPING CONTAINER 

COOLANT 

DAYS
TESTED 

TURN-
AROUND 

COMMENTS 

Acid Fast Stain

8.50

1) affected tissue
2) smear or slide
3) swab

1) sterile container
2) slide folder
3) culturette

1,3) frozen gel packs
2) none

M-F

1 day


Actinobacillus pleuropneumoniae - APP (PCR)
31.50
1) tonsillar swab
2) whole tonsil
3) lung
1) swab
2,3) tissue
frozen gel packs M 2-10 days Results reported on Tuesdays.

Additional info.
Actinobacillus pleuropneumoniae - APP (PCR) Serotypes 3, 6 and 8
31.50
1) tonsillar swab
2) whole tonsil
3) lung
1) swab
2,3) tissue
frozen gel packs M-F 2-10 days Testing upon request.
Actinobacillus pleuropneumoniae/ Actinobacillus suis Multiplex-APP/ A. suis (PCR)
31.50
1) tonsillar swab
2) whole tonsil
3) lung
1) swab
2,3) tissue
frozen gel packs M 2-10 days Results reported on Tuesdays.
Actinobacillus pleuropneumoniae - APP Serotyping (PCR )
31.50
bacterial isolate culture media frozen gel packs T 2-10 days Results are reported on Wednesdays. (Rush testing available upon request)
Actinobacillus suis (PCR)
31.50

1) tonsillar swab
2) whole tonsil
3) lung

1) swab
2,3) tissue
frozen gel packs M 2-10 days

Results reported on Tuesdays.

Additional info.

Aerobic Culture 

17.75

1) swab
2) affected tissue

1) culturette
2) sterile container

frozen gel packs

M-F

2-5  days

Samples should be kept cool and moist in transit.

Anaerobic Culture 

19.50

1) swab
2) affected tissue

1) anaerobic transport media
2) sterile container

1) none
2) frozen gel packs

M-F

2-5 days

Ship overnight.

Aquaculture Bacterial Inspection

90.00 per lot (up to 60)
live fish leakproof container

1) frozen gel packs
2) none

M-W 10 days See "Aquaculture/ Fisheries Submission Form" for details on tests and pathogens. Does not include Renibacterium salmoninarum IFAT.
Bacterial identification by DNA sequencing (16S rRNA gene)
26.25
bacterial isolate culture media frozen gel packs Th 1-2 weeks

Results reported on Thursdays.

Additional info.

Bacterial isolate send out 

78.75

isolate






Biotyping, Pasteurella multocida 

16.75

pure culture

agar plate or slant

frozen gel packs

M-F

7 days

Prevent agar from freezing.

Blood Culture, Aerobic 

22.25

5 ml blood

100 ml blood culture bottles

none

M-F

2-7 days


Blood Culture, Anaerobic 

24.25

5 ml blood

100 ml blood culture bottles

none

M-F

3-10 days


Brachyspira spp. Culture 

22.25

1) fresh colon section
2) fresh feces

1,2) sterile container

frozen gel packs

M-F

10-14 days

Ship overnight or deliver the same day.

Brachyspira hyodysenteriae and Brachyspira pilosicoli multiplex PCR  

31.50

bacterial isolate

culture media

frozen gel packs

M

2-10 days

Results are reported on Tuesdays.

Campylobacter Culture, Bovine Reproduction 

27.50

1) aborted fetus
2) prepucial  flush
3) cervical mucous

1) sterile container
2,3) Campy  transport media

frozen gel packs

M-F

6-14  days

Transport media is supplied by the laboratory.

Campylobacter Culture, Small Animal 

27.50

1) feces
2) intestine (tied loop)

1,2) sterile container

frozen gel packs

M-F

6-9  days

Use anaerobic transport media if delayed >24 hr.

Campylobacter Screen 

10.00






Part of necropsy.

Chlamydia (Chlamydophila) Antigen Capture (ELISA) 

25.25

swabs

plastic tube

frozen gel packs

M-F

2-3 days


Chlamydia (Chlamydophila) Isolation (Cell culture) 

31.25

feces in Chlamydia transport media

sealed container

frozen gel packs

Th

2-3 weeks

Contact lab for transport media.

Clostridium difficile (ELISA) 

13.50

1) 1 gm intestinal contents, or
2) 1 gm of feces

1,2) plastic bag or tube

frozen gel packs

once weekly

2-7 days

Tests run when sufficient number of samples are available.

Clostridium perfringens toxin gene typing (PCR) 

28.50

bacterial isolate

culture plate

frozen gel packs

T

2-10 days

Results reported on Wednesdays.

Dark Field Microscopy for Brachyspira/ Spirochetes 

7.25

1) spiral colon
2) fresh feces

1,2) sterile container

frozen gel packs

M-F

1 day

Ship overnight   or deliver the same day.

Dark Field Microscopy for Leptospira 

7.75

urine

sterile container

frozen gel packs

M-F

1 day

Ship overnight or deliver the same day.

Dark Field Microscopy for Tritrichomonas 

7.75

1) prepucial flush
2) cervical mucus

1,2) sterile container

frozen gel packs

M-F

1 day

Ship overnight   or deliver the same day.

Environmental Culture 

27.50

swab

culturette

frozen gel packs

M-F

2-5  days

Samples should be kept cool and moist in transit.
Erysipelothrix rhusiopathiae (PCR)
31.50
1) skin lesions
2) systemic tissues
3) fetuses
tissue frozen gel packs M 2-10 days Results reported on Tuesdays.

Additional info.
Escherichia coli Virulence Factors
27.75
bacterial isolate culture media frozen gel packs T 2-10 days Results reported on Wednesdays.

Fluorescent Antibody, Clostridia spp. (chauvoei, novyi, sordellii, septicum) 

13.50

1) affected tissue
2) 5 thin smears

1) sterile container
2) slide folder

1) frozen gel packs
2) none

M-F

1 day

Fix slides in acetone for 5 minutes.

Fluorescent Antibody, Leptospira spp. 

8.75

10 ml urine, midstream

redtop vacutainer , not a serum separation tube

frozen gel packs

T,Th

4-5 days

Do NOT freeze. No formalin.

Fungal Culture (dermatophytes)  

18.25

1) affected tissue
2) hair or scraping
3) swab

1) sterile container
2) envelope
3) culturette

1,3) frozen gel packs
2) none

M-F

4 weeks


Fungal Screen (rapid growing fungi-yeast)  

6.25

1) affected tissue
2) swab

1) sterile container
2) culturette

frozen gel packs

M-F

2 days


Fungal Stain (fluorescent and KOH) 

12.25

1) affected tissue
2) smear or slide
3) swab
4) hair sample

1) sterile container
2) slide folder
3) culturette
4) envelope

1,3) frozen gel packs
2,4) none

M-F

1 day


Genotype analysis, bacterial (BioNumerics)
31.50
bacterial isolate culture media frozen gel packs W,Th 1-2 weeks Results reported on Tuesdays.

Giemsa Stain 

7.75

1) tissue
2) fluid
3) slide

1,2) sterile container
3) slide folder

1,2) frozen gel packs
3) none

M-F

1 day


Gimenez Stain 

11.75

1) tissue
2) fluid
3) slide

1,2) sterile container
3) slide folder

1,2) frozen gel packs
3) none

M-F

1 day


Gram Stain 

7.75

1) tissue
2) fluid
3) slide

1,2) sterile container
3) slide folder

1,2) frozen gel packs
3) none

M-F

1 day


Haemophilus parasuis (PCR)
31.50
systemic tissues (fibrin) 1) swabs
2) tissues
3) fluids
frozen gel packs M,Th 2-10 days Results reported on Tuesdays and Fridays.

Additional info.

Isolate Identification 

1) 27.50
2) 38.00

1) pure culture
2) impure culture

1,2) agar plate or slant

none

M-F

5  days

Prevent agar from freezing.

Lawsonia intracellularis (PCR), equine 

30.00

1) ileum
2) feces

1,2) sterile container

frozen gel packs

M-F

7 days


Lawsonia intracellularis (PCR), porcine 

30.00

1) ileum
2) feces

1,2) sterile container

frozen gel packs

M-F

7 days


Leptospira sp. (PCR)
15.75
1) 10 ml urine
2) tissue (fetuses, kidney)
3) 100 ml water

1) vial
2) plastic bag
3) leakproof container

frozen gel packs W 2-10 days Results reported on Fridays.

Listeria Culture 

19.75

1) tissue
2) silage

1) sterile container
2) clean, sealed container

1) frozen gel packs
2) none

M-F

4-30 days


Mycobacterium paratuberculosis - Johne's Culture 

25.00

five grams of feces

tightly covered, clean container

frozen gel packs

M-F

16 weeks

Please call to schedule.

Mycobacterium paratuberculosis - Johne's (PCR) 

20.00

feces (no more than 10 g needed)

Do NOT use GLOVE or WHIRL PAC BAG as container.

Use leakproof hard plastic container with screw cap

frozen gel packs or dry ice

T,Th,Fr

3 days-1 week

Please make sure container has no outside contamination. NO GLOVES or WHIRL PAC BAGS. Specimens from sheep, goat and exotic ruminants also accepted.

Mycoplasma hyopneumoniae (PCR)

25.25

1) swab (nasal, tracheal or bronchial)
2) lung

1) culturette
2) whirl pac or plastic bag

frozen gel packs

F

same day


Mycoplasma hyopneumoniae Genetic Analysis (P146 Adhesin sequencing)

126.00

1) swabs (nasal, tracheal, or bronchial)*
2) lung*
3) isolate

*Positive for M. hyo. by PCR

1) synthetic swab
2) whirl pac or plastic bag
3) sterile tube

frozen gel packs

weekly

2-4 weeks

1) Extracted DNA can be sent in FTA paper for testing.
2) Must come with a copy of USDA shipping permit.
3) Shipping instruction: send next day delivery

Mycoplasma hyorhinis (PCR) 

15.75

1) joint swab
2) joint fluid
3) synovial membrane
1) swab
2) tissue
frozen gel packs M 2-10 days Results reported on Tuesdays.

Mycoplasma hyosynoviae (PCR) 

15.75

1) joint swab
2) joint fluid
3) synovial membrane
1) swab
2) tissue
frozen gel packs M 2-10 days Results reported on Tuesdays.

Mycoplasma speciation

26.25

bacterial isolate culture media frozen gel packs Th 1-2 weeks

Results reported on Thursdays.

Additional info

Mycoplasma spp. Culture 

22.25

1) uterine, cervical or vaginal mucus
2) semen
3) lung tissue
4) tracheal wash
5) fluid

1,2,4,5) Amies transport media
3) sterile container

frozen gel packs

M-F

14 days

Ship overnight or deliver the same day.

Pasteurella multocida Toxin Testing (PCR) 

31.50

1) nasal swabs
2) P. multocida isolate

1) swab
2) culture media

frozen gel pack

M

2-10 days

Results reported on Tuesdays. (Rush testing available)

Renibacterium salmoninarum (IFA) 

100.00 per lot (60 fish)

live fish

leakproof container

1) frozen gel packs
2) none

M-W

3-5 days


Salmonella enterica Real-time PCR (TaqMan® )

26.25

1) tissue
2) feces (no more than 10g needed)

Do NOT use GLOVE or WHIRL PAC BAG as container.

Use leakproof hard plastic container with screw cap

frozen gel packs or dry ice

Th

3-7 days

Please make sure container has no outside contamination. NO GLOVES or WHIRL PAC BAGS.

Salmonella spp. Culture 

17.75

1) tissue
2) feces

1,2) clean container

frozen gel packs

M-F

2-5 days


Salmonella spp. Screen (Delayed Enrichment) 

22.25

1) chicks
2) eggs
3) feed
4) environmental samples

1,2,3,4) clean container (well labeled)

1) frozen gel packs
2,3,4) none

M-F

5-14 days

Prevent eggs from freezing or breaking.

Salmonella spp. Serogrouping 

6.25

pure culture

agar plate or slant

frozen gel pack

M-F

1 day

Prevent agar from freezing

Salmonella spp. Serotyping 

No charge for diagnostic isolates

(see comments)

pure culture

agar plate or slant

frozen gel pack

M-F

4-6 weeks

Prevent agar from freezing.Note: $36.00 charge for research and environmental isolates.

Serotyping (Actinobacillus pleuropneumoniae, Streptococcus suis, Haemophilus parasuis

Reference lab fee

pure culture

agar plate or slant

frozen gel pack

M-F

2-3 weeks

Prevent agar from freezing.

Streptococcus suis PCR

31.50

systemic tissues

1) swabs
2) tissues
3) fluids

frozen gel packs

F

2-10 days

Results are reported on Mondays.

Streptococcus suis serotyping PCR (serotypes 1,2,7 and 9)

31.50

bacterial isolate

culture media

frozen gel packs

M

2-10 days

Results are reported on Tuesdays.

Susceptibility Testing 

16.75

1) tissue
2) swab
3) pure culture

1) sterile container
2) culturette
3) agar plate or slant

frozen gel packs

M-F

2-4 days


Tritrichomonas spp. Culture 

15.50

1) prepucial flush
2) cervical mucus

1,2) Trichomonas transport media

none

M-F

4 days

Transport media is furnished by the lab.

Ureaplasma spp. Culture 

22.25

1) uterine, cervical or vaginal mucus
2) semen
3) lung tissue
4) tracheal wash
5) fluid

1,2,4,5) Amies transport media
3) sterile container

frozen gel packs

M-F

7 days

Ship overnight or deliver the same day.

Urine Culture 

17.75

urine collected by cystocentesis

syringe or sterile tube

frozen gel packs

M-F

2-3 days

Qualitative and quantitative cultures will be done.

Urolith Culture 

60.25

urolith

clean container

none

M-F

3 days


Water Culture (includes counts on total Gram negative, Gram negative lactose fermenters (coliforms), Gram negative non-lactose fermenters, and Pseudomonas aeruginosa

32.75

200 ml water

sterile container

frozen gel packs

M-F

4 days

Keep chilled.

Yersinia Culture 

22.25

1) feces
2) tissue

1,2) sterile container

frozen gel packs

M-F

2-4 days


 

 


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